Molecular group:
Immune cells
Molecule/Cell:
CD4+CD25+Foxp3+ Treg cells
Plasmodium strain:
Plasmodium berghei ANKA
pRBC infection titer:
10^5
Mouse genetic background (Con):
C57BL/6
Experimental treatment group (EG):
anti-CD25 (day -14)
Lethal infection (Con):
yes
Lethal infection (EG):
yes
Pathology (Con):
CM (100%)
Pathology (EG):
No CM
Parasitemia (EG vs Con):
Reduced on day 5
Additional phenotypes (EG vs Con):
Prolonged survival (2 weeks) (possible effect of inducible Treg cells??); no neurological symptoms; parasite burden is much higher than estimated by blood smears due to accumulation of parasites in peripheral tissue and vasculature; parasite burden greatly (~10-fold) decreased by anti-CD25; Brain: ↓ pRBC accumulation; similar VCAM-1 and ICAM-1; ↓ CD8+ T cells, ↑ number of macrophages and similar CD4+ T, B, NK, NKT cells, PMN, DC and microglia; similar CD69+CD4+ cells; ↓ CD69+CD8+ T cells; Spleen:↑ CD69+CD4+ and CD69+CD8+ T cells; ↑ IFN-g+ CD4+ and similar IFN-g+CD8+ cells (day 5); similar IFN-g and IL-10 mRNA (day 5); Lymph nodes: CD69+CD4+ and CD69+CD8+ T cells; Blood: ↓ CD69+CD4+ cells; ↓ CD69+CD8+ T cells (no difference between CD69+CD25+ and CD69+CD25- T cells → no depletion of activated CD25+CD4+ or CD25+ CD8+ T cells); ↑ MCP-1, ↓ IL-10 and similar TNF-a and IL-6 (day 5)
Refs:
Amante et al., 2007, Am J Pathol
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