Molecular group:
Leucocyte effector mechanisms and proteases
Molecule/Cell:
Histamine R3
Plasmodium strain:
Plasmodium berghei ANKA
pRBC infection titer:
10^6
Mouse genetic background (Con):
C57BL/6J x 129/Ola
Level of backcrossing:
N.I.
Experimental treatment group (EG):
H3R -/-
Lethal infection (Con):
yes
Lethal infection (EG):
yes
Pathology (Con):
CM (100%)
Pathology (EG):
CM (100%)
Parasitemia (EG vs Con):
Increased
Additional phenotypes (EG vs Con):
Earlier death (days); Brain: earlier and ↑ Evans blue dye extravasation; ↑ number of RBC (u+p) aggregates; ↑ sequestration of CD4+, CD8+ T cells, CD11b+GR1low (macrophages) and CD11b+GR1high (Neutrophils + inflammatory macrophages) cells; ↑ levels of tele-methylhistamine (also in non-infected H3R-/- mice); ↓ mRNA expression of histidine decarboxylase (HDC) 6 days p.i. (naïve H3R -/- mice have higher levels than naïve C56BL/6J mice); ↑ plasma histamine levels 3 and 5 days p.i. (not 6 days p.i.); in vitro IL-10 and TNF-a production by P. berghei ANKA stimulated splenocytes peaked with a delay of 1 day, while IFN-g production was ↑ 4 days p.i.
Refs:
Beghdadi et al., 2009, PLoS One
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