Histamine R3

Molecular group: 
Leucocyte effector mechanisms and proteases
Molecule/Cell: 
Histamine R3
Plasmodium strain: 
Plasmodium berghei ANKA
pRBC infection titer: 
10^6
Mouse genetic background (Con): 
C57BL/6J x 129/Ola
Level of backcrossing: 
N.I.
Experimental treatment group (EG): 
H3R -/-
Lethal infection (Con): 
yes
Lethal infection (EG): 
yes
Pathology (Con): 
CM (100%)
Pathology (EG): 
CM (100%)
Parasitemia (EG vs Con): 
Increased
Additional phenotypes (EG vs Con): 
Earlier death (days); Brain: earlier and ↑ Evans blue dye extravasation; ↑ number of RBC (u+p) aggregates; ↑ sequestration of CD4+, CD8+ T cells, CD11b+GR1low (macrophages) and CD11b+GR1high (Neutrophils + inflammatory macrophages) cells; ↑ levels of tele-methylhistamine (also in non-infected H3R-/- mice); ↓ mRNA expression of histidine decarboxylase (HDC) 6 days p.i. (naïve H3R -/- mice have higher levels than naïve C56BL/6J mice); ↑ plasma histamine levels 3 and 5 days p.i. (not 6 days p.i.); in vitro IL-10 and TNF-a production by P. berghei ANKA stimulated splenocytes peaked with a delay of 1 day, while IFN-g production was ↑ 4 days p.i.
Refs: 
Beghdadi et al., 2009, PLoS One