Molecular group:
Leucocyte effector mechanisms and proteases
Molecule/Cell:
Perforin (PFP)
Plasmodium strain:
Plasmodium berghei ANKA
pRBC infection titer:
10^6
Mouse genetic background (Con):
C57BL/6
Level of backcrossing:
N.I.
Experimental treatment group (EG):
PFP -/-
Lethal infection (Con):
yes
Lethal infection (EG):
yes
Pathology (Con):
CM (100%)
Pathology (EG):
No CM
Parasitemia (EG vs Con):
N.I.
Additional phenotypes (EG vs Con):
Prolonged survival (2-3 weeks); similar edema, sequestration of pRBCs and accumulation of CD44+CD62L- CD4 and CD8 T cells in the brain; adoptively transfered splenocytes from infected WT mice migrate to the brain and induce CM; adoptively transfered CD8+ cells from infected WT mice, but not from infected PFP -/- mice, induce CM in RAG2 -/- mice (lack peripheral T and B cells); ↓ cytolytic activity of IFN-g producing CD8+ cells; similar expression of CXCR3, CXCR4, CCR2 and CCR5 on CD8+ cells
Refs:
Nitcheu et al., 2003, J Immunol
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