Molecular group:
Cytokines and receptors
Molecule/Cell:
IFN-gR
Plasmodium strain:
Plasmodium berghei ANKA
pRBC infection titer:
5 x 10^5 or 10^6
Mouse genetic background (Con):
129/Sv/Ev x C57BL/6 (WT = 129 Sv/Ev)
Level of backcrossing:
Mixed background (50%)
Experimental treatment group (EG):
IFN-gR -/-
Lethal infection (Con):
yes
Lethal infection (EG):
yes
Pathology (Con):
CM (60-100% depending on the parasite dose used)
Pathology (EG):
No CM
Parasitemia (EG vs Con):
Similar/higher depending on the parasite dose used
Additional phenotypes (EG vs Con):
No neurological symptoms; similar anemia; ↑ serum IFN-g, TNF-a; similar IFN-g protein expression in the brain and ↑ IFN-g protein expression in the spleen; ↓ TNF-a mRNA expression in the spleen and brain; ↓ TNF-a protein expression in the brain; ↓ IL-6 and IL-10 mRNA expression in the brain; similar IL-6 mRNA expression in the spleen; similar NO in serum, brain and spleen
Refs:
Amani et al., 2000, Eur J Immunol
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