Molecular group:
Cytokines and receptors
Molecule/Cell:
IL-12p40
Plasmodium strain:
Plasmodium chabaudi chabaudi AS
pRBC infection titer:
10^6 (primary infection); 10^9 (secondary challenge)
Mouse genetic background (Con):
129/Sv x C57BL/6J
Level of backcrossing:
Backcrossed for 5 generations
Experimental treatment group (EG):
IL-12p40 -/-
Lethal infection (Con):
no
Lethal infection (EG):
no
Pathology (Con):
N.I.
Pathology (EG):
N.I.
Parasitemia (EG vs Con):
Increased first peak and developed a prominent second peak
Additional phenotypes (EG vs Con):
Similar protection during second challenge; first peak (day 7): no NO or IFN-g production by spleen cells, no NO secretion after IFN-g stimulation of spleen cells; second peak (day 15): no NO production by spleen cells, similar NO secretion after IFN-g stimulation of spleen cells;↓ NO and ↑ TGF-b1 production by macrophages after stimulation with pRBCs or rIFN-g; ↓ internalization of pRBCs coated with immune serum antibodies by macrophages
Refs:
Bastos et al., 2002, J Interferon Cytokine Res
Hyperlink: