Molecular group:
Cytokines and receptors
Molecule/Cell:
IL-3
Plasmodium strain:
Plasmodium berghei NK65
pRBC infection titer:
10^5
Mouse genetic background (Con):
BALB/c
Level of backcrossing:
Backcrossed for 4 generations
Experimental treatment group (EG):
IL-3 -/-
Lethal infection (Con):
yes
Lethal infection (EG):
yes
Pathology (Con):
N.I.
Pathology (EG):
N.I.
Parasitemia (EG vs Con):
♂ Increased on day 8-10; ♀ Similar
Additional phenotypes (EG vs Con):
♂ Prolonged survival (2-3 weeks); ♀ Similar mortality (♀ infected WT mice survive 2-3 weeks longer than ♂ infected WT mice); ↑ splenomegaly and anemia; ↑ numbers of BFU-E and CFU-E in bone marrow and spleen; similar ↑ plasma levels of GM-CSF, IL-1a, IL-1b, IL-6, IL-9, IL-10, IL-12p70, IL-13, IP-10, KC, MCP-1, M-CSF, MIP-1a, MIP-1b, and TNF; plasma levels of IFN-g and MIG are lower on day 4 and similarly ↑ on day 8 p.i., whereas G-CSF levels are higher on day 4 and 8 p.i.; ↓ % of blood neutrophils at day 8 p.i.
Refs:
Auclair et al., 2013, Infect Immun
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